DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Jong-Young | ko |
dc.contributor.author | Kim, Yong-Tae | ko |
dc.contributor.author | Ahn, Jin-Woo | ko |
dc.contributor.author | Kim, Kwan-Suk | ko |
dc.contributor.author | Gweon, Dae-Gab | ko |
dc.contributor.author | Seo, Tae-Seok | ko |
dc.date.accessioned | 2013-03-12T10:13:05Z | - |
dc.date.available | 2013-03-12T10:13:05Z | - |
dc.date.created | 2012-07-06 | - |
dc.date.created | 2012-07-06 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.35, no.1, pp.327 - 334 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/102009 | - |
dc.description.abstract | An integrated allele-specific (AS) polymerase chain reaction (PCR) and capillary electrophoresis (CE) microdevice has been developed for multiplex single nucleotide polymorphism (SNP) genotyping on a portable instrumentation, which was applied for on-site identification of HANWOO (Korean indigenous beef cattle). Twelve sets of primers were designed for targeting beef cattle's eleven SNP loci for HANWOO verification and one primer set for a positive PCR control, and tie success rate for identification of HANWOO was demonstrated statistically. The AS PCR and CE separation for multiplex SNP typing was carried out on a glass-based microchip consisting of four layers: a microchannel plate for microfluidic control, a Pt-electrode plate for a resistance temperature detector (RID), a poly(dimethylsiloxane) (PDMS) membrane and a manifold glass for microvalve function. The operation of the sample loading, AS PCR, microvalve, and CE on a chip was automated with a portable genetic analyzer, and the laser-induced fluorescence detection was performed on a miniaturized fluorescence detector. The blind samples were correctly identified as a HANWOO by showing one or two amplicon peaks in the electropherogram, while the imported beef cattle revealed more than five peaks. Our genetic analysis platform provides rapid, accurate, and on-site multiplex SNP typing. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | DNA | - |
dc.subject | SNP | - |
dc.subject | PCR | - |
dc.subject | MARKERS | - |
dc.title | Integrated allele-specific polymerase chain reaction-capillary electrophoresis microdevice for single nucleotide polymorphism genotyping | - |
dc.type | Article | - |
dc.identifier.wosid | 000305036000050 | - |
dc.identifier.scopusid | 2-s2.0-84860515117 | - |
dc.type.rims | ART | - |
dc.citation.volume | 35 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 327 | - |
dc.citation.endingpage | 334 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2012.03.009 | - |
dc.contributor.localauthor | Gweon, Dae-Gab | - |
dc.contributor.localauthor | Seo, Tae-Seok | - |
dc.contributor.nonIdAuthor | Kim, Kwan-Suk | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Integrated microdevice | - |
dc.subject.keywordAuthor | Single nucleotide polymorphism | - |
dc.subject.keywordAuthor | Allele-specific polymerase chain reaction | - |
dc.subject.keywordAuthor | Capillary electrophoresis | - |
dc.subject.keywordAuthor | On-site detection | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SNP | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | MARKERS | - |
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