Metabolic engineering of Escherichia coli for the production of 1-propanol

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An engineered Escherichia coli strain that produces 1-propanol under aerobic condition was developed based on an L-threonine-overproducing E. coil strain. First, a feedback resistant ilvA gene encoding threonine dehydratase was introduced and the competing metabolic pathway genes were deleted. Further engineering was performed by overexpressing the cimA gene encoding citramalate synthase and the ackA gene encoding acetate kinase A/propionate kinase II, introducing a modified adhE gene encoding an aerobically functional AdhE, and by deleting the rpoS gene encoding the stationary phase sigma factor. Fed-batch culture of the final engineered strain harboring pBRthrABC-tac-cimA-tac-ackA and pTacDA-tac-adhE(mut) allowed production of 10.8 g L-1 of 1-propanol with the yield and productivity of 0.107 g g(-1) and 0.144 g L-1 h(-1), respectively, from 100 g L-1 of glucose, and 10.3 g L-1 of 1-propanol with the yield and productivity of 0.259 g g(-1) and 0.083 g L-1 h(-1), respectively, from 40 g L-1 glycerol. (C) 2012 Elsevier Inc. All rights reserved.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2012-09
Language
English
Article Type
Article
Citation

METABOLIC ENGINEERING, v.14, no.5, pp.477 - 486

ISSN
1096-7176
DOI
10.1016/j.ymben.2012.07.006
URI
http://hdl.handle.net/10203/101917
Appears in Collection
CBE-Journal Papers(저널논문)
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