Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing

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Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2 x 10(9) to 8 x 10(9) (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from similar to 2 x 10(6) to similar to 5 x 10(6) (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000 +/- 45 mg/kg to below 5 mg/kg. While 99.9%, i.e. up to below 5 mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel. (C) 2012 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE INC
Issue Date
2012-05
Language
English
Article Type
Article
Keywords

CONTAMINATED SOIL; BIOREMEDIATION; BIOAUGMENTATION; REMEDIATION; OIL

Citation

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.18, no.3, pp.882 - 887

ISSN
1226-086X
DOI
10.1016/j.jiec.2012.01.020
URI
http://hdl.handle.net/10203/101699
Appears in Collection
CBE-Journal Papers(저널논문)
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