DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, TJ | ko |
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Pan, JG | ko |
dc.contributor.author | Jung, HC | ko |
dc.contributor.author | Park, JY | ko |
dc.contributor.author | Park, JP | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-03-11T02:13:24Z | - |
dc.date.available | 2013-03-11T02:13:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.citation | LETTERS IN APPLIED MICROBIOLOGY, v.53, no.4, pp.445 - 451 | - |
dc.identifier.issn | 0266-8254 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98015 | - |
dc.description.abstract | Aims: The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin-displayed spores as a molecular machinery. Methods and Results: Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of Bacillus thuringiensis spores and as a diagnosis method for DNA detection. As a proof-of-concept, four pathogenic bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Klebsiella pneumonia were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson's disease were also used for the detection of single nucleotide polymorphism (SNP) in this system. Conclusions: This strategy, utilizing streptavidin-displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson's disease. Significance and Impact of the Study: This approach could be useful as a simple platform for developing sensitive spore-based biosensors for any desired DNA targets in diagnostic applications. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | DNA capturing machinery through spore-displayed proteins | - |
dc.type | Article | - |
dc.identifier.wosid | 000295095600009 | - |
dc.identifier.scopusid | 2-s2.0-80052639552 | - |
dc.type.rims | ART | - |
dc.citation.volume | 53 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 445 | - |
dc.citation.endingpage | 451 | - |
dc.citation.publicationname | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Pan, JG | - |
dc.contributor.nonIdAuthor | Jung, HC | - |
dc.contributor.nonIdAuthor | Park, JY | - |
dc.contributor.nonIdAuthor | Park, JP | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | pathogenic bacteria | - |
dc.subject.keywordAuthor | single nucleotide polymorphism detection | - |
dc.subject.keywordAuthor | spore display | - |
dc.subject.keywordAuthor | streptavidin | - |
dc.subject.keywordAuthor | Wilson&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordPlus | BACTERIAL SURFACE DISPLAY | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | WILSON-DISEASE | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | STREPTAVIDIN | - |
dc.subject.keywordPlus | FREQUENCY | - |
dc.subject.keywordPlus | VECTOR | - |
dc.subject.keywordPlus | GENE | - |
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