The transcription unit architecture of the Escherichia coli genome

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Bacterial genomes are organized by structural and functional elements, including promoters, transcription start and termination sites, open reading frames, regulatory noncoding regions, untranslated regions and transcription units. Here, we iteratively integrate high-throughput, genome-wide measurements of RNA polymerase binding locations and mRNA transcript abundance, 5' sequences and translation into proteins to determine the organizational structure of the Escherichia coli K-12 MG1655 genome. Integration of the organizational elements provides an experimentally annotated transcription unit architecture, including alternative transcription start sites, 5' untranslated region, boundaries and open reading frames of each transcription unit. A total of 4,661 transcription units were identified, representing an increase of >530% over current knowledge. This comprehensive transcription unit architecture allows for the elucidation of condition-specific uses of alternative sigma factors at the genome scale. Furthermore, the transcription unit architecture provides a foundation on which to construct genome-scale transcriptional and translational regulatory networks.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2009-11
Language
English
Article Type
Article
Keywords

RNA-POLYMERASE; ACCURATE MASS; ANNOTATION; PROTEOMICS; PROTEIN; K-12; RECONSTRUCTION; EXPRESSION; INITIATION; NETWORKS

Citation

NATURE BIOTECHNOLOGY, v.27, no.11, pp.1043 - 115

ISSN
1087-0156
DOI
10.1038/nbt.1582
URI
http://hdl.handle.net/10203/97453
Appears in Collection
BS-Journal Papers(저널논문)
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