DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Byoung-Sam | ko |
dc.contributor.author | Kim, Dong-Min | ko |
dc.contributor.author | Yoon, Byoung-Hoon | ko |
dc.contributor.author | Bai, Suk | ko |
dc.contributor.author | Lee, Hyeon-Yong | ko |
dc.contributor.author | Kim, Jung-Hoe | ko |
dc.contributor.author | Kim, Il-Chul | ko |
dc.date.accessioned | 2013-03-09T07:59:23Z | - |
dc.date.available | 2013-03-09T07:59:23Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.33, no.6, pp.1209 - 1213 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/95801 | - |
dc.description.abstract | To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | GENE-DISRUPTED MUTANT | - |
dc.subject | D-XYLOSE | - |
dc.subject | YIELD | - |
dc.title | Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis | - |
dc.type | Article | - |
dc.identifier.wosid | 000291655200019 | - |
dc.identifier.scopusid | 2-s2.0-79955942312 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1209 | - |
dc.citation.endingpage | 1213 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.contributor.nonIdAuthor | Ko, Byoung-Sam | - |
dc.contributor.nonIdAuthor | Kim, Dong-Min | - |
dc.contributor.nonIdAuthor | Bai, Suk | - |
dc.contributor.nonIdAuthor | Lee, Hyeon-Yong | - |
dc.contributor.nonIdAuthor | Kim, Il-Chul | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Candida tropicalis | - |
dc.subject.keywordAuthor | Enzyme engineering | - |
dc.subject.keywordAuthor | Fermentation | - |
dc.subject.keywordAuthor | Xylitol | - |
dc.subject.keywordAuthor | Xylitol dehydrogenase | - |
dc.subject.keywordPlus | GENE-DISRUPTED MUTANT | - |
dc.subject.keywordPlus | D-XYLOSE | - |
dc.subject.keywordPlus | YIELD | - |
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