Spore Display Using Bacillus thuringiensis Exosporium Protein InhA

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and P-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2009-05
Language
ENG
Keywords

BACTERIAL SURFACE DISPLAY; SUBTILIS SPORES; CELL-SURFACE; HETEROLOGOUS PROTEINS; ANTHRACIS EXOSPORIUM; METALLOPROTEASE; IDENTIFICATION; LIBRARIES; ANTIGENS; CEREUS

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.5, pp.495 - 501

ISSN
1017-7825
DOI
10.4014/jmb.0802.163
URI
http://hdl.handle.net/10203/9396
Appears in Collection
CBE-Journal Papers(저널논문)
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