DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, JY | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.contributor.author | Jung, SO | ko |
dc.contributor.author | Park, J | ko |
dc.date.accessioned | 2013-03-08T10:28:23Z | - |
dc.date.available | 2013-03-08T10:28:23Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.33, no.2, pp.19 - 26 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92833 | - |
dc.description.abstract | In the present study, we exploited the superior features of peptide nucleic acids (PNAs) to develop an efficient PNA zip-code microarray for the detection of hepatocyte nuclear factor-1alpha (HNF-1alpha) mutations that cause type 3 maturity onset diabetes of the young (MODY). A multi-epoxy linker compound was synthesized and used to achieve an efficient covalent linking of amine-modified PNA to an aminated glass surface. PCR was performed to amplify the genomic regions containing the mutation sites. The PCR products were then employed as templates in a subsequent multiplex single base extension reaction using chimeric primers with 3' complementarity to the specific mutation site and 5' complementarity to the respective PNA zip-code sequence on the microarray. The primers were extended by a single base at each corresponding mutation site in the presence of biotin-labeled ddNTPs, and the products were hybridized to the PNA microarray. Compared to the corresponding DNA, the PNA zip-code sequence showed a much higher duplex specificity for the complementary DNA sequence. The PNA zip-code microarray was finally stained with streptavidin-R-phycoerythrin to generate a fluorescent signal. Using this strategy, we were able to correctly diagnose several mutation sites in exon 2 of HNF-1alpha with a wild-type and mutant samples including a MODY3 patient. This work represents one of the few successful applications of PNA in DNA chip technology. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | PEPTIDE NUCLEIC-ACID | - |
dc.subject | DNA MICROARRAYS | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | POLYMORPHISM | - |
dc.subject | ARRAYS | - |
dc.subject | YOUNG | - |
dc.subject | CONFORMATION | - |
dc.subject | ABUNDANCE | - |
dc.subject | SAMPLES | - |
dc.subject | GENE | - |
dc.title | Diagnosis of HNF-1 alpha mutations on a PNA zip-code microarray by single base extension | - |
dc.type | Article | - |
dc.identifier.wosid | 000226941000009 | - |
dc.identifier.scopusid | 2-s2.0-19744383318 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 19 | - |
dc.citation.endingpage | 26 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/gni020 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Song, JY | - |
dc.contributor.nonIdAuthor | Jung, SO | - |
dc.contributor.nonIdAuthor | Park, J | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PEPTIDE NUCLEIC-ACID | - |
dc.subject.keywordPlus | DNA MICROARRAYS | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | POLYMORPHISM | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.subject.keywordPlus | YOUNG | - |
dc.subject.keywordPlus | CONFORMATION | - |
dc.subject.keywordPlus | ABUNDANCE | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.subject.keywordPlus | GENE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.