Binding and enrichment of Escherichia coli spheroplasts expressing inner membrane tethered scFv antibodies on surface immobilized antigens

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Anchored periplasmic expression (APEx) is a new method for the isolation of, high affinity ligand-binding proteins from large combinatorial libraries (Harvey et al, 2004, Proc Natl Acad Sci USA 101(25): 9193-9198). In APEx, proteins are expressed as fusions to a membrane anchor that tethers them onto the periplasmic side of the Escherichia coli inner membrane. Conversion of the cells into spheroplasts and incubation with soluble fluorescently conjugated ligands results in the specific labeling of cells expressing ligand-binding proteins and their subsequent isolation by flow cytometry. Here we show that scFv antibody fragments expressed in the APEx format allow the binding of spheroplasts to immobilized ligands. ScFv antibodies specific for the cardiac glycoside digoxin or for the protective antigen (PA) of Bacillus anthracis as a negative control were expressed in E. coli as fusions to either N-terminal or C-terminal membrane anchoring domains. Only the C-terminally anchored fusions resulted in specific recognition and binding, of spheroplasts onto TentaGel beads with immobilized antigen. Following three rounds of flow cytometric screening, spheroplasts expressing anti-digoxin scFvs were enriched 950-fold from a large excess (1,000x ) of spheroplasts expressing anti-PA antibodies. These results indicate that the APEx technology may be employed for the screening of libraries based on binding to insoluble antigens possibly including antigens on cell surfaces.
Publisher
JOHN WILEY SONS INC
Issue Date
2007-09
Language
English
Article Type
Article
Keywords

COMBINATORIAL LIBRARIES; TARGETING PEPTIDE; DISPLAY; AFFINITY; PROTEIN; PHAGE; YEAST; MUTAGENESIS; FRAGMENTS; VECTORS

Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.98, no.1, pp.39 - 47

ISSN
0006-3592
DOI
10.1002/bit.21405
URI
http://hdl.handle.net/10203/90666
Appears in Collection
CBE-Journal Papers(저널논문)
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