Determination of volatile components of pine needles from Pinus densiflora S. using solid phase trapping extraction-gas chromatography-mass spectrometry

Abstract

Volatile components of the needles from Pinus densiflora were studied by gas chromatography-mass spectrometry (GC-MS) applying a new solid phase trapping extraction (SPTE) method and a Magic Chemisorber (TM) (MC). In SPTE, a titanium tube of the surface covered With polydimethylsiloxane (PDMS) was used. The efficiency of SPTE method was studied by varying extraction temperature (20, 35, 50 and 65 degrees C) at a fixed extraction time of 20 min. The optimum temperature was 35 degrees C. Extraction efficiency of SPTE method was conpared to that of SPME method under the same optimum conditions (extraction time 20 min, extraction temperature 35 degrees C). For SPTE, extraction efficiency of hydrocarbons increased with the increasing temperature. However, alcohols and carbonyls, such as 2-hexenal, 1-hexanol, and cis-3-hexenol were extracted less efficiently at higher temperatures. Fatty acids, such as capric, lauric, myristic, pentadecanoic, and palmitic were identified by SPTE, but not by SPME under the same (SPTE optimum) experimental conditions.