DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jungwoon | ko |
dc.contributor.author | Rhee, Byung Kirl | ko |
dc.contributor.author | Bae, Gab-Yong | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.contributor.author | Kim, Jungho | ko |
dc.date.accessioned | 2013-03-07T14:54:56Z | - |
dc.date.available | 2013-03-07T14:54:56Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-06 | - |
dc.identifier.citation | STEM CELLS, v.23, no.6, pp.738 - 751 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90436 | - |
dc.description.abstract | The Oct-4 gene encodes a transcription factor that is expressed in embryonic stem (ES) cells and germ cells. Oct-4 is known to function as a transcriptional activator of genes involved in maintaining an undifferentiated totipotent state and possibly in preventing expression of genes activated during differentiation. In addition, it is a putative proto-oncogene and a critical player in the genesis of human testicular germ cell tumors. Although much effort has gone toward characterizing Oct-4, there is still little known about the molecular mechanisms and the proteins that regulate Oct-4 function. To identify cofactors that control Oct-4 function in vivo, we used a recently developed bacterial two-hybrid screening system and isolated a novel ES cell-derived cDNA encoding Ewing's sarcoma protein (EWS). EWS is a proto-oncogene and putative RNA-binding protein involved in human cancers. By using glutathione-S-transferase (GST) pull-down assays, we were able to confirm the interaction between Oct-4 and EWS in vitro, and moreover, coimmunoprecipitation and colocalization studies have shown that these proteins also associate in vivo. We have mapped the EWS-interacting region to the POU domain of Oct-4. In addition, three independent sites on EWS are involved in binding to Oct-4. In this study, we report that Oct-4 and EWS are coexpressed in the pluripotent mouse and human ES cells. Consistent with its ability to bind to and colocalize with Oct-4, ectopic expression of EWS enhances the transactivation ability of Oct-4. Moreover, a chimeric protein generated by fusion of EWS (1-295) to the GAL4 DNA-binding domain significantly increases promoter activity of a reporter containing GAL4 DNA-binding sites, suggesting the presence of a strong activation domain within EWS. Taken together, our results suggest that Oct-4-mediated transactivation is stimulated by EWS. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | RNA-POLYMERASE-II | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | HUMAN CHORIONIC-GONADOTROPIN | - |
dc.subject | TRANSCRIPTION FACTOR OCT-3/4 | - |
dc.subject | PRIMORDIAL GERM-CELLS | - |
dc.subject | CREB-BINDING PROTEIN | - |
dc.subject | BREAST-CANCER CELLS | - |
dc.subject | POU-DOMAIN | - |
dc.subject | MOUSE EMBRYOGENESIS | - |
dc.subject | MOLECULAR-GENETICS | - |
dc.title | Stimulation of Oct-4 activity by Ewings sarcoma protein | - |
dc.type | Article | - |
dc.identifier.wosid | 000229625100004 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 738 | - |
dc.citation.endingpage | 751 | - |
dc.citation.publicationname | STEM CELLS | - |
dc.identifier.doi | 10.1634/stemcells.2004-0375 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Lee, Jungwoon | - |
dc.contributor.nonIdAuthor | Rhee, Byung Kirl | - |
dc.contributor.nonIdAuthor | Bae, Gab-Yong | - |
dc.contributor.nonIdAuthor | Kim, Jungho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Oct-4 | - |
dc.subject.keywordAuthor | Ewing&apos | - |
dc.subject.keywordAuthor | s sarcoma protein | - |
dc.subject.keywordAuthor | proto-oncogene | - |
dc.subject.keywordAuthor | transcriptional coactivator protein-protein interaction | - |
dc.subject.keywordAuthor | bacterial two-hybrid screening | - |
dc.subject.keywordAuthor | embryonic stem cells | - |
dc.subject.keywordPlus | RNA-POLYMERASE-II | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | HUMAN CHORIONIC-GONADOTROPIN | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR OCT-3/4 | - |
dc.subject.keywordPlus | PRIMORDIAL GERM-CELLS | - |
dc.subject.keywordPlus | CREB-BINDING PROTEIN | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | POU-DOMAIN | - |
dc.subject.keywordPlus | MOUSE EMBRYOGENESIS | - |
dc.subject.keywordPlus | MOLECULAR-GENETICS | - |
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