Hydrogen production from Chlamydomonas reinhardtii biomass using a two-step conversion process: Anaerobic conversion and photosynthetic fermentation

Cited 105 time in webofscience Cited 0 time in scopus
  • Hit : 240
  • Download : 0
Chlamydomonas reinhardtii UTEX 90 accumulated 1.45 g dry cell weight and 0.77 g starch/L during photosynthetic growth using TAP media at 25 degrees C in presence of 2% CO2 for 3 days. C. reinhardtii biomass was concentrated and then converted into hydrogen and organic acids by anaerobic fermentation with Clostridium butyricum. Organic acids in the fermentate of algal biomass were consecutively photo-dissimilated to hydrogen by Rhodobacter sphaeroides KD131. In the concentrated algal biomass 52% of the starch was hydrolyzed to 37.1 mmol H-2/L-concentrated algal biomass and 13.6, 25.5, 7.4 and 493 mM of formate, acetate, propionate, and butyrate, respectively by C. butyricum. R. sphaeroides KD131 evolved 5.72 mmol H-2 per ml-fermentate of algal biomass under illumination of 8 klux at 30 degrees C. Only 80% of the organic acids, mainly butyrate, were hydrolyzed during photo-incubation. During anaerobic conversion, 2.58 mol H-2/mol starch-glucose was evolved using C. butyricum and then 5.72 mol H-2/L-anaerobic fermentate was produced by R. sphaeroides KD131. Thus, the two-step conversion process produced 8.30 mol H-2 from 1 mol starch-glucose equivalent algal biomass via organic acids. 0 2005 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
2006-05
Language
English
Article Type
Article
Keywords

BACTERIA; PHOTOPRODUCTION

Citation

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.31, no.6, pp.812 - 816

ISSN
0360-3199
DOI
10.1016/j.ijhydene.2005.06.009
URI
http://hdl.handle.net/10203/90041
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 105 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0