Click chemistry to construct fluorescent oligonucleotides for DNA sequencing

Click chemistry 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with singlebase resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
Publisher
AMER CHEMICAL SOC
Issue Date
2003-01
Language
ENG
Keywords

CUCURBITURIL; CYCLOADDITION; CATALYSIS; PRIMERS

Citation

JOURNAL OF ORGANIC CHEMISTRY, v.68, no.2, pp.609 - 612

ISSN
0022-3263
DOI
10.1021/jo026615r
URI
http://hdl.handle.net/10203/8856
Appears in Collection
CBE-Journal Papers(저널논문)
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