Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production

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dc.contributor.authorLee, Jun Hyoungko
dc.contributor.authorSung, Bong Hyunko
dc.contributor.authorKim, Mi Sunko
dc.contributor.authorBlattner, Frederick R.ko
dc.contributor.authorYoon, Byoung Hoonko
dc.contributor.authorKim, Jung Hoeko
dc.contributor.authorKim, Sun-Changko
dc.date.accessioned2009-04-13T05:56:08Z-
dc.date.available2009-04-13T05:56:08Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2009-12-
dc.identifier.citationMICROBIAL CELL FACTORIES, v.8-
dc.identifier.issn1475-2859-
dc.identifier.urihttp://hdl.handle.net/10203/8739-
dc.description.abstractBackground: Deletion of large blocks of nonessential genes that are not needed for metabolic pathways of interest can reduce the production of unwanted by-products, increase genome stability, and streamline metabolism without physiological compromise. Researchers have recently constructed a reduced-genome Escherichia coli strain MDS42 that lacks 14.3% of its chromosome. Results: Here we describe the reengineering of the MDS42 genome to increase the production of the essential amino acid L-threonine. To this end, we over-expressed a feedback-resistant threonine operon (thrA*BC), deleted the genes that encode threonine dehydrogenase (tdh) and threonine transporters (tdcC and sstT), and introduced a mutant threonine exporter (rhtA23) in MDS42. The resulting strain, MDS-205, shows an similar to 83% increase in L-threonine production when cells are grown by flask fermentation, compared to a wild- type E. coli strain MG1655 engineered with the same threonine-specific modifications described above. And transcriptional analysis revealed the effect of the deletion of non-essential genes on the central metabolism and threonine pathways in MDS-205. Conclusion: This result demonstrates that the elimination of genes unnecessary for cell growth can increase the productivity of an industrial strain, most likely by reducing the metabolic burden and improving the metabolic efficiency of cells.-
dc.description.sponsorshipThis work was supported in part by grants from 21C Frontier Program of Microbial Genomics and Applications (MG08-0204-1-0), the Research Program of New Drug Target Discovery (M10748222314-08N4800-31410) from the Ministry of Education, Science and Technology, the Korea Science and Engineering Foundation Grant (R01-2008-000-20559-0), and the Korea Research Foundation Grant (KRF-2004-042-D00072).en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherBIOMED CENTRAL LTD-
dc.subjectRECOMBINANT PROTEIN-PRODUCTION-
dc.subjectPHOSPHOENOLPYRUVATE CARBOXYKINASE-
dc.subjectGENE-EXPRESSION-
dc.subjectSUCCINIC ACID-
dc.subjectFERMENTATION-
dc.subjectIMPROVEMENT-
dc.subjectSYSTEM-
dc.subjectK-12-
dc.subjectCELL-
dc.subjectSET-
dc.titleMetabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production-
dc.typeArticle-
dc.identifier.wosid000263588100001-
dc.type.rimsART-
dc.citation.volume8-
dc.citation.publicationnameMICROBIAL CELL FACTORIES-
dc.identifier.doi10.1186/1475-2859-8-2-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorKim, Jung Hoe-
dc.contributor.localauthorKim, Sun-Chang-
dc.contributor.nonIdAuthorLee, Jun Hyoung-
dc.contributor.nonIdAuthorSung, Bong Hyun-
dc.contributor.nonIdAuthorKim, Mi Sun-
dc.contributor.nonIdAuthorBlattner, Frederick R.-
dc.type.journalArticleArticle-
dc.subject.keywordPlusRECOMBINANT PROTEIN-PRODUCTION-
dc.subject.keywordPlusPHOSPHOENOLPYRUVATE CARBOXYKINASE-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusSUCCINIC ACID-
dc.subject.keywordPlusFERMENTATION-
dc.subject.keywordPlusIMPROVEMENT-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusK-12-
dc.subject.keywordPlusCELL-
dc.subject.keywordPlusSET-
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