DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Jae Seung | ko |
dc.contributor.author | Kim, Hye Jin | ko |
dc.contributor.author | Ryu, Young Hee | ko |
dc.contributor.author | Yun, Cheol-Heui | ko |
dc.contributor.author | Chung, Dae Kyun | ko |
dc.contributor.author | Han, Seung Hyun | ko |
dc.date.accessioned | 2013-03-06T15:08:48Z | - |
dc.date.available | 2013-03-06T15:08:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | JOURNAL OF IMMUNOLOGY, v.176, pp.S35 - S35 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87364 | - |
dc.description.abstract | Commercially available pokeweed mitogen (PWM) has been reported to activate macrophages, leading to production of proinflammatory cytokines and nitric oxide (NO). However, we found that polymyxin B (PMB), a specific inhibitor of endotoxin activity, inhibited the PWM-induced expression of proinflammatory cytokines and NO and the activation of Toll-like receptor 4 (TLR4). A kinetic-turbidimetric Limulus amebocyte lysate assay demonstrated that commercial PWM contained substantial endotoxin, over 104 endotoxin units/mg of the PWM. A PWM re purified by PMB-coupled beads no longer induced the expression of proinflammatory cytokines, TLR4 activation, or dendritic cell maturation. However, the repurified PWM remained able to induce proliferation of human lymphocytes, which is a representative characteristic of PWM. These results suggest that commercial PWM might be contaminated with a large amount of endotoxin, resulting in the attribution of misleading immunological properties to PWM. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.title | Endotoxin contamination in commercially available pokeweed mitogen contributes to the activation of murine macrophages and human dendritic cell maturation | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-33645537080 | - |
dc.type.rims | ART | - |
dc.citation.volume | 176 | - |
dc.citation.beginningpage | S35 | - |
dc.citation.endingpage | S35 | - |
dc.citation.publicationname | JOURNAL OF IMMUNOLOGY | - |
dc.identifier.doi | 10.1128/CVI.13.3.309-313.2006 | - |
dc.contributor.localauthor | Yang, Jae Seung | - |
dc.contributor.nonIdAuthor | Kim, Hye Jin | - |
dc.contributor.nonIdAuthor | Ryu, Young Hee | - |
dc.contributor.nonIdAuthor | Yun, Cheol-Heui | - |
dc.contributor.nonIdAuthor | Chung, Dae Kyun | - |
dc.contributor.nonIdAuthor | Han, Seung Hyun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Meeting Abstract | - |
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