DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, JM | ko |
dc.contributor.author | Shin, YB | ko |
dc.contributor.author | Kim, MG | ko |
dc.contributor.author | Ro, HS | ko |
dc.contributor.author | Jung, HeeTae | ko |
dc.contributor.author | Chung, BH | ko |
dc.date.accessioned | 2013-03-05T02:12:00Z | - |
dc.date.available | 2013-03-05T02:12:00Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-07 | - |
dc.identifier.citation | ANALYTICAL BIOCHEMISTRY, v.330, no.2, pp.251 - 256 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | http://hdl.handle.net/10203/84953 | - |
dc.description.abstract | A surface plasmon resonance (SPR) imaging system was constructed and used to detect the affinity-tagged recombinant proteins expressed in Escherichia coli. With regards to model proteins, the hexahistidine-ubiquitin-tagged human growth hormone (His(6)-Ub-hGH), glutathione S-transferase-tagged human interleukin-6 (GST-hIL6), and maltose-binding protein-tagged human interleukin-6 (MBP-hIL6) expressed in E. coli were analyzed. The cell lysates were spotted on gold thin films coated with 11-mercaptoundecanol (MUOH)/dextran derivatized with Ni(II)-iminodiacetic acid (IDA-Ni(II)), glutathione, or cyclodextrin. After a brief washing of the gold chip, SPR imaging measurements were carried out in order to detect the bound affinity-tagged fusion proteins. Using this new approach, rapid high-throughput expression analysis of the affinity-tagged proteins were obtained. The SPR imaging protein chip system used to measure the expression of affinity-tagged proteins in a high-throughput manner is expected to be an attractive alternative to traditional laborious and time-consuming methods, such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | Academic Press Inc Elsevier Science | - |
dc.subject | ARRAYS | - |
dc.title | A fusion protein expression analysis using surface plasmon resonance imaging | - |
dc.type | Article | - |
dc.identifier.wosid | 000222424800009 | - |
dc.identifier.scopusid | 2-s2.0-2942625909 | - |
dc.type.rims | ART | - |
dc.citation.volume | 330 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 251 | - |
dc.citation.endingpage | 256 | - |
dc.citation.publicationname | ANALYTICAL BIOCHEMISTRY | - |
dc.identifier.doi | 10.1016/j.ab.2004.02.009 | - |
dc.contributor.localauthor | Jung, HeeTae | - |
dc.contributor.nonIdAuthor | Jung, JM | - |
dc.contributor.nonIdAuthor | Shin, YB | - |
dc.contributor.nonIdAuthor | Kim, MG | - |
dc.contributor.nonIdAuthor | Ro, HS | - |
dc.contributor.nonIdAuthor | Chung, BH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | affinity-tagged protein | - |
dc.subject.keywordAuthor | expression analysis | - |
dc.subject.keywordAuthor | gold chip | - |
dc.subject.keywordAuthor | surface plasmon resonance (SPR) imaging | - |
dc.subject.keywordPlus | ARRAYS | - |
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