A fusion protein expression analysis using surface plasmon resonance imaging

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dc.contributor.authorJung, JMko
dc.contributor.authorShin, YBko
dc.contributor.authorKim, MGko
dc.contributor.authorRo, HSko
dc.contributor.authorJung, HeeTaeko
dc.contributor.authorChung, BHko
dc.date.accessioned2013-03-05T02:12:00Z-
dc.date.available2013-03-05T02:12:00Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-07-
dc.identifier.citationANALYTICAL BIOCHEMISTRY, v.330, no.2, pp.251 - 256-
dc.identifier.issn0003-2697-
dc.identifier.urihttp://hdl.handle.net/10203/84953-
dc.description.abstractA surface plasmon resonance (SPR) imaging system was constructed and used to detect the affinity-tagged recombinant proteins expressed in Escherichia coli. With regards to model proteins, the hexahistidine-ubiquitin-tagged human growth hormone (His(6)-Ub-hGH), glutathione S-transferase-tagged human interleukin-6 (GST-hIL6), and maltose-binding protein-tagged human interleukin-6 (MBP-hIL6) expressed in E. coli were analyzed. The cell lysates were spotted on gold thin films coated with 11-mercaptoundecanol (MUOH)/dextran derivatized with Ni(II)-iminodiacetic acid (IDA-Ni(II)), glutathione, or cyclodextrin. After a brief washing of the gold chip, SPR imaging measurements were carried out in order to detect the bound affinity-tagged fusion proteins. Using this new approach, rapid high-throughput expression analysis of the affinity-tagged proteins were obtained. The SPR imaging protein chip system used to measure the expression of affinity-tagged proteins in a high-throughput manner is expected to be an attractive alternative to traditional laborious and time-consuming methods, such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots. (C) 2004 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.publisherAcademic Press Inc Elsevier Science-
dc.subjectARRAYS-
dc.titleA fusion protein expression analysis using surface plasmon resonance imaging-
dc.typeArticle-
dc.identifier.wosid000222424800009-
dc.identifier.scopusid2-s2.0-2942625909-
dc.type.rimsART-
dc.citation.volume330-
dc.citation.issue2-
dc.citation.beginningpage251-
dc.citation.endingpage256-
dc.citation.publicationnameANALYTICAL BIOCHEMISTRY-
dc.identifier.doi10.1016/j.ab.2004.02.009-
dc.contributor.localauthorJung, HeeTae-
dc.contributor.nonIdAuthorJung, JM-
dc.contributor.nonIdAuthorShin, YB-
dc.contributor.nonIdAuthorKim, MG-
dc.contributor.nonIdAuthorRo, HS-
dc.contributor.nonIdAuthorChung, BH-
dc.type.journalArticleArticle-
dc.subject.keywordAuthoraffinity-tagged protein-
dc.subject.keywordAuthorexpression analysis-
dc.subject.keywordAuthorgold chip-
dc.subject.keywordAuthorsurface plasmon resonance (SPR) imaging-
dc.subject.keywordPlusARRAYS-
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