DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이현호 | ko |
dc.contributor.author | 김상준 | ko |
dc.contributor.author | 신현재 | ko |
dc.contributor.author | 박지연 | ko |
dc.contributor.author | 양지원 | ko |
dc.date.accessioned | 2013-03-04T23:41:13Z | - |
dc.date.available | 2013-03-04T23:41:13Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-12 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.7, no.6, pp.375 - 379 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/84673 | - |
dc.description.abstract | Methyl mercaptan oxidase was successfully induced in Thiobacillus thioparus TK-m using methyl mercaptan gas, and was purified for the detection of mercaptans. The purification procedure Involved a DEAE (diethylaminoethyl) -Sephacel, or Superose 12, column chromatography with recovery yields of 47.5 and 48.5%, and specific activities of 374 and 1240.8 units/mg-protein, respectively, The molecular weight of the purified methyl mercaptan oxidase was 66.1kDa, as determined by SDS-PAGE. The extract, from gel filtration chromatography oxidizes methyl mercaptan, producing formaldehyde, which can be easily detected by the purpald-coloring method. The optimized temperature for activity was found to be at 55$\^{C}$. This enzyme was inhibited by both NH4Cl and (NH4)2SO4, but was unaffected by either KCl or NaCl at less than 200 mM. With K2SO4, the activity decreased at 20 mM, but recovered at 150 mM. In the presence of methanol, full activity was maintained, but decreased in the presence of glycerin, ethanol and acetone 43, 78 and 75%, respectively. | - |
dc.language | English | - |
dc.publisher | 한국생물공학회 | - |
dc.title | Purification and Characterization of Methyl Mercaptan Oxidase from Thiobacillus thioparus for Mercaptan Detection | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 375 | - |
dc.citation.endingpage | 379 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.kciid | ART001211630 | - |
dc.contributor.localauthor | 양지원 | - |
dc.contributor.nonIdAuthor | 이현호 | - |
dc.contributor.nonIdAuthor | 김상준 | - |
dc.contributor.nonIdAuthor | 신현재 | - |
dc.contributor.nonIdAuthor | 박지연 | - |
dc.subject.keywordAuthor | methyl mercaptan oxidase | - |
dc.subject.keywordAuthor | Thiobacillus thioparus | - |
dc.subject.keywordAuthor | purpald | - |
dc.subject.keywordAuthor | enzyme purification | - |
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