DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tomita, Kazunori | ko |
dc.contributor.author | Kibe, Tatsuya | ko |
dc.contributor.author | Kang, Ho-Young | ko |
dc.contributor.author | Seo, Yeon-Soo | ko |
dc.contributor.author | Uritani, Masahiro | ko |
dc.contributor.author | Ushimaru, Takashi | ko |
dc.contributor.author | Ueno, Masaru | ko |
dc.date.accessioned | 2013-03-04T15:21:06Z | - |
dc.date.available | 2013-03-04T15:21:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-11 | - |
dc.identifier.citation | MOLECULAR AND CELLULAR BIOLOGY, v.24, no.21, pp.9557 - 9567 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | http://hdl.handle.net/10203/83053 | - |
dc.description.abstract | It has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-rich strand. Here, we demonstrate that a taz1-d dna2-2C double mutant lost the G-rich overhang at a semipermissive temperature. The amount of G-rich overhang in S phase in the dna2-C2 mutant was lower than that in wild-type cells at the semipermissive temperature. Dna2 bound to telomere DNA in a chromatin immunoprecipitation assay. Moreover, telomere length decreased with each generation after shift of the dna2-2C mutant to the semipermissive temperature. These results suggest that Dna2 is involved in the generation of G-rich overhangs in both wild-type cells and taz1-d cells. The dna2-C2 mutant was not gamma ray sensitive at the semipermissive temperature, suggesting that the ability to process double-strand break (DSB) ends was not affected in the dna2-C2 mutant. Our results reveal that DSB ends and telomere ends are processed by different mechanisms. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | OKAZAKI FRAGMENT MATURATION | - |
dc.subject | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | POLYMERASE ALPHA | - |
dc.subject | S-PHASE | - |
dc.subject | CATALYTIC SUBUNIT | - |
dc.subject | KU HETERODIMER | - |
dc.subject | BUDDING YEAST | - |
dc.subject | G TAILS | - |
dc.subject | REPLICATION | - |
dc.title | Fission yeast Dna2 is required for generation of the telomeric single-strand overhang | - |
dc.type | Article | - |
dc.identifier.wosid | 000224943300027 | - |
dc.identifier.scopusid | 2-s2.0-6344284126 | - |
dc.type.rims | ART | - |
dc.citation.volume | 24 | - |
dc.citation.issue | 21 | - |
dc.citation.beginningpage | 9557 | - |
dc.citation.endingpage | 9567 | - |
dc.citation.publicationname | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.identifier.doi | 10.1128/MCB.24.21.9557-9567.2004 | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.nonIdAuthor | Tomita, Kazunori | - |
dc.contributor.nonIdAuthor | Kibe, Tatsuya | - |
dc.contributor.nonIdAuthor | Kang, Ho-Young | - |
dc.contributor.nonIdAuthor | Uritani, Masahiro | - |
dc.contributor.nonIdAuthor | Ushimaru, Takashi | - |
dc.contributor.nonIdAuthor | Ueno, Masaru | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | OKAZAKI FRAGMENT MATURATION | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | POLYMERASE ALPHA | - |
dc.subject.keywordPlus | S-PHASE | - |
dc.subject.keywordPlus | CATALYTIC SUBUNIT | - |
dc.subject.keywordPlus | KU HETERODIMER | - |
dc.subject.keywordPlus | BUDDING YEAST | - |
dc.subject.keywordPlus | G TAILS | - |
dc.subject.keywordPlus | REPLICATION | - |
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