Surface fabrication of biotinylated polypeptide multi-layer designed for surface plasmon resonance sensing

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The novel modification of gold surfaces with a biotinylated polypeptide multilayer formed by nonspecific electrostatic adsorption was designed for the SPR sensing platform. The multilayer of polypeptides (poly-L-lysine and poly(aspartic acid)) was prepared onto a negatively charged self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) on gold film. The polypeptide multilayer was functionalized with biotin by directly grafting to poly (succinimide) (PSI). The specific binding of avidin to the biotinylated polypeptide multilayer (DS 2.7%) resulted in a shift in the refractive index of 0.003 +/- 9 x 10(-4) with a surface density of 2.9 ng/mm(2). This multilayer surface was found to provide a simple and effective method for the SPR sensing.
Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
Issue Date
2004-12
Language
English
Article Type
Article; Proceedings Paper
Keywords

KINETIC-ANALYSIS; BIOSENSOR

Citation

JOURNAL OF NONLINEAR OPTICAL PHYSICS & MATERIALS, v.13, pp.525 - 534

ISSN
0218-8635
URI
http://hdl.handle.net/10203/82691
Appears in Collection
CBE-Journal Papers(저널논문)
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