DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Hae-Jeon | ko |
dc.contributor.author | Yoon, Sang-Hyun | ko |
dc.contributor.author | Lee, Mee-Jeong | ko |
dc.contributor.author | Kim, Myo-Jeong | ko |
dc.contributor.author | Kweon, Ki-Sung | ko |
dc.contributor.author | Lee, In-Won | ko |
dc.contributor.author | Kim, Jung-Wan | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Lee, Hyun-Soo | ko |
dc.contributor.author | Spiridonova, Vera A. | ko |
dc.contributor.author | Park, Kwan-Hwa | ko |
dc.date.accessioned | 2013-03-03T07:14:45Z | - |
dc.date.available | 2013-03-03T07:14:45Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-03 | - |
dc.identifier.citation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.46, no.3, pp.952 - 959 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77737 | - |
dc.description.abstract | A thermostable cyclodextrin glucanotransferase (CGTase) was isolated from a Bacillus stearothermophilus strain, ET1, which was screened from Korean soil. The corresponding CGTase gene cloned in Escherichia coli shared 84% and 88% identity with CGTase genes from other B. stearothermophilus strains at the nucleotide and amino acid sequence level, respectively. The enzyme was purified to apparent homogeneity by beta-cyclodextrin (CD) affinity chromatography and high-performance liquid chromatography. The enzyme had an apparent molecular mass of 66,800 Da and a pI of 5.0. The optimum pH for the enzyme-catalyzed reaction was pH 6.0, and the optimum temperature was observed at 80 degrees C. Thermostability of the enzyme was enhanced by Ca2+, A 13% (w/v) cornstarch solution was liquefied and converted to CDs solely using this enzyme. The cornstarch conversion rate was 44% and alpha-, beta-, and gamma-CDs were produced in the ratio of 4.2:5.9:1. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CYCLOMALTODEXTRIN-GLUCANOTRANSFERASE | - |
dc.subject | ALKALOPHILIC-BACILLUS | - |
dc.subject | BETA-CYCLODEXTRIN | - |
dc.subject | PURIFICATION | - |
dc.subject | GLYCOSYLTRANSFERASE | - |
dc.subject | ENZYME | - |
dc.subject | TRANSFERASE | - |
dc.subject | AMYLASE | - |
dc.title | Characterization of a thermostable cyclodextrin glucanotransferase isolated from Bacillus stearothermophilus ET1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000072624200026 | - |
dc.identifier.scopusid | 2-s2.0-0000738026 | - |
dc.type.rims | ART | - |
dc.citation.volume | 46 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 952 | - |
dc.citation.endingpage | 959 | - |
dc.citation.publicationname | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.identifier.doi | 10.1021/jf970707d | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Chung, Hae-Jeon | - |
dc.contributor.nonIdAuthor | Yoon, Sang-Hyun | - |
dc.contributor.nonIdAuthor | Lee, Mee-Jeong | - |
dc.contributor.nonIdAuthor | Kim, Myo-Jeong | - |
dc.contributor.nonIdAuthor | Kweon, Ki-Sung | - |
dc.contributor.nonIdAuthor | Lee, In-Won | - |
dc.contributor.nonIdAuthor | Kim, Jung-Wan | - |
dc.contributor.nonIdAuthor | Lee, Hyun-Soo | - |
dc.contributor.nonIdAuthor | Spiridonova, Vera A. | - |
dc.contributor.nonIdAuthor | Park, Kwan-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cyclodextrin glucanotransferase | - |
dc.subject.keywordAuthor | thermostability | - |
dc.subject.keywordAuthor | cyclodextrin | - |
dc.subject.keywordAuthor | Bacillus stearothermophilus | - |
dc.subject.keywordPlus | CYCLOMALTODEXTRIN-GLUCANOTRANSFERASE | - |
dc.subject.keywordPlus | ALKALOPHILIC-BACILLUS | - |
dc.subject.keywordPlus | BETA-CYCLODEXTRIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | GLYCOSYLTRANSFERASE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | TRANSFERASE | - |
dc.subject.keywordPlus | AMYLASE | - |
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