Optimization of culture conditions for production of a high viscosity polysaccharide, methylan, by methylobacterium organophilum from methanol

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dc.contributor.authorChoi J.H.ko
dc.contributor.authorKim, Jung Hoeko
dc.contributor.authorKim S.Y.ko
dc.contributor.authorOh D.Kko
dc.date.accessioned2013-03-03T04:45:34Z-
dc.date.available2013-03-03T04:45:34Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1998-06-
dc.identifier.citationKOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.3, pp.244 - 249-
dc.identifier.issn0257-2389-
dc.identifier.urihttp://hdl.handle.net/10203/77283-
dc.description.abstractAn extracellular polysaccharide, methylan, was produced under the specific conditions by Methylobacteriurn organophilum from methanol. The specific growth rate of cells was approximately constant regardless of C/N ratio and the specific product yield was maximum at a C/N ratio of 30. Methylan production was suppressed by the deficiency of mineral ions such as Mn** or Fe** ion. The optimal pH for cell growth and methylan production was 7. Whereas the optimal temperature for cell growth was found to be 37°C, that for methylan production was 30°C. The methanol concentration above 4% completely inhibited the cell growth. The initial methanol concentration for the maximal production of methylan was 0.5% (v/v) and above this concentration, methylan production was markedly inhibited. To overcome the substrate toxicity and inhibition for both cell growth and methylan production, a fed-bach culture of intermittent feeding within 5 g/l methanol was conducted under the optimal culture condition. Methylan production of was stimulated by nitrogen limitation and methylan was accumulated up to 8.7 g/l and cell mass also increased up to 12.4 g/l.-
dc.languageEnglish-
dc.publisherKorean Society for Applied Microbiology-
dc.titleOptimization of culture conditions for production of a high viscosity polysaccharide, methylan, by methylobacterium organophilum from methanol-
dc.typeArticle-
dc.identifier.scopusid2-s2.0-0031847818-
dc.type.rimsART-
dc.citation.volume26-
dc.citation.issue3-
dc.citation.beginningpage244-
dc.citation.endingpage249-
dc.citation.publicationnameKOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.localauthorKim, Jung Hoe-
dc.contributor.nonIdAuthorChoi J.H.-
dc.contributor.nonIdAuthorKim S.Y.-
dc.contributor.nonIdAuthorOh D.K-
dc.subject.keywordAuthorMethanol-
dc.subject.keywordAuthorMethylobacterium organophilum-
dc.subject.keywordAuthorOptimization of culture condition-
dc.subject.keywordAuthorPolysaccharide-methylan-
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