A lipopeptide biosurfactant produced by Bacillus subtilis C9 selected through the oil film-collapsing assay

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Bacillus subtilis C9 was selected by measuring the oil film-collapsing activity and produced biosurfactant in a medium containing glucose as a sole carbon source. The biosurfactant emulsified hydrocarbons, vegetable oils and crude oil, and lowered the surface tension of culture broth to 28 dyne/cm. A biosurfactant, C9-BS produced by B. subtilis C9 was purified by ultrafiltration, extraction with chloroform and methanol, adsorption chromatography, and preparative reversed phase HPLC. Structural analyses, IR spectroscopy, FAB mass spectroscopy, amino acid composition, and NMR analyses, demonstrated that CB-BS was a lipopeptide comprising a fatty acid tail and peptide moiety, The Lipophilic part consisting of C-14 or C-15 hydroxy fatty acid was linked to the hydrophilic peptide part, which contained seven amino acids (Glu-Leu-Leu-Val-Asp-Leu-Leu) with a lactone linkage.
Publisher
KOREAN SOC APPLIED MICROBIOLOGY
Issue Date
1997-06
Language
English
Article Type
Article
Keywords

RECOVERY MEOR; MICROORGANISMS; JF-2

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.7, no.3, pp.180 - 188

ISSN
1017-7825
URI
http://hdl.handle.net/10203/76907
Appears in Collection
CBE-Journal Papers(저널논문)
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