DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Dae-Sik | ko |
dc.contributor.author | Vogel, Hannes | ko |
dc.contributor.author | Willerford, Dennis M. | ko |
dc.contributor.author | Sands, Arthur T. | ko |
dc.contributor.author | Platt, Kenneth A. | ko |
dc.contributor.author | Hasty, Paul | ko |
dc.date.accessioned | 2013-03-02T18:01:50Z | - |
dc.date.available | 2013-03-02T18:01:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-06 | - |
dc.identifier.citation | MOLECULAR AND CELLULAR BIOLOGY, v.20, no.11, pp.3772 - 3780 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74825 | - |
dc.description.abstract | Absence of Ku80 results in increased sensitivity to ionizing radiation, defective lymphocyte development, early onset of an age-related phenotype, and premature replicative senescence. Here we investigate the role of p53 on the phenotype of ku80-mutant mice and cells. Reducing levels,of p53 increased the cancer incidence for ku80(-/-) mice. About 20% of ku80(-/-) p53(+/-) mice developed a broad spectrum of cancer by 40 weeks and all ku80(-/-) p53(-/-) mice developed pro-B-cell lymphoma by 16 weeks. Reducing levels of p53 rescued populations of ku80(-/-) cells from replicative senescence by enabling spontaneous immortalization. The double-mutant cells are impaired for the G(1)/S checkpoint due to the p53 mutation and are hypersensitive to gamma-radiation and reactive oxygen species due to the Ku80 mutation. These data show that replicative senescence is caused by a p53-dependent cell cycle response to damaged DNA in ku80(-/-) cells and that p53 is essential for preventing very early onset of pro-B-cell lymphoma in ku80(-/-) mice. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | SEVERE COMBINED IMMUNODEFICIENCY | - |
dc.subject | STRAND BREAK REPAIR | - |
dc.subject | X-RAY RESISTANCE | - |
dc.subject | V(D)J RECOMBINATION | - |
dc.subject | DNA-REPAIR | - |
dc.subject | KU86-DEFICIENT MICE | - |
dc.subject | P53-DEFICIENT MICE | - |
dc.subject | SCID MUTATION | - |
dc.subject | PROTEIN | - |
dc.subject | CANCER | - |
dc.title | Analysis of ku80-mutant mice and cells with deficient levels of p53 | - |
dc.type | Article | - |
dc.identifier.wosid | 000087017100003 | - |
dc.identifier.scopusid | 2-s2.0-0034106125 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 3772 | - |
dc.citation.endingpage | 3780 | - |
dc.citation.publicationname | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.identifier.doi | 10.1128/MCB.20.11.3772-3780.2000 | - |
dc.contributor.localauthor | Lim, Dae-Sik | - |
dc.contributor.nonIdAuthor | Vogel, Hannes | - |
dc.contributor.nonIdAuthor | Willerford, Dennis M. | - |
dc.contributor.nonIdAuthor | Sands, Arthur T. | - |
dc.contributor.nonIdAuthor | Platt, Kenneth A. | - |
dc.contributor.nonIdAuthor | Hasty, Paul | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SEVERE COMBINED IMMUNODEFICIENCY | - |
dc.subject.keywordPlus | STRAND BREAK REPAIR | - |
dc.subject.keywordPlus | X-RAY RESISTANCE | - |
dc.subject.keywordPlus | V(D)J RECOMBINATION | - |
dc.subject.keywordPlus | DNA-REPAIR | - |
dc.subject.keywordPlus | KU86-DEFICIENT MICE | - |
dc.subject.keywordPlus | P53-DEFICIENT MICE | - |
dc.subject.keywordPlus | SCID MUTATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CANCER | - |
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