DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jaehoon | ko |
dc.contributor.author | Bae, Sung Won | ko |
dc.contributor.author | Hong, Hyo Jeong | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2013-03-02T17:51:11Z | - |
dc.date.available | 2013-03-02T17:51:11Z | - |
dc.date.created | 2012-04-03 | - |
dc.date.created | 2012-04-03 | - |
dc.date.issued | 1996-08 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.51, no.4, pp.479 - 487 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74795 | - |
dc.description.abstract | The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) Of transfectoma decreased by 60% to 88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the q(Ab) of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the q(Ab) of P at the beginning of long-term culture, the q(Ab) of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased q(Ab) of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased q(Ab) of P during long-term culture. (C) 1996 John Wiley & Sons, Inc. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY SONS INC | - |
dc.subject | HYBRIDOMA CELL-LINE | - |
dc.subject | HEPATITIS-B VIRUS | - |
dc.subject | SERUM-FREE MEDIA | - |
dc.subject | STABILITY | - |
dc.subject | ANTIGEN | - |
dc.subject | MOUSE | - |
dc.subject | SPECIFICITY | - |
dc.subject | SECRETION | - |
dc.subject | MYELOMAS | - |
dc.subject | CLONING | - |
dc.title | Decreased chimeric antibody productivity of KR12H-1 transfectoma during long-term culture results from decreased antibody gene copy number | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UY51600011 | - |
dc.identifier.scopusid | 2-s2.0-9544246862 | - |
dc.type.rims | ART | - |
dc.citation.volume | 51 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 479 | - |
dc.citation.endingpage | 487 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
dc.contributor.nonIdAuthor | Bae, Sung Won | - |
dc.contributor.nonIdAuthor | Hong, Hyo Jeong | - |
dc.contributor.nonIdAuthor | Lee, Gyun Min | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | transfectoma | - |
dc.subject.keywordAuthor | chimeric antibody | - |
dc.subject.keywordAuthor | stability | - |
dc.subject.keywordAuthor | Southern blot | - |
dc.subject.keywordPlus | HYBRIDOMA CELL-LINE | - |
dc.subject.keywordPlus | HEPATITIS-B VIRUS | - |
dc.subject.keywordPlus | SERUM-FREE MEDIA | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | MYELOMAS | - |
dc.subject.keywordPlus | CLONING | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.