Flow cytometric analysis of antibody producing cells using double immunofluorescent staining

Double fluorescent labeling, with fluorescein isothiocyanate (FITC)-labeled F(ab')(2) specific for the heavy chain and R-phycoerythrin (R-PE)-labcled F(ab')(2) specific for the light chain, was demonstrated as a convenient means for the accurate evaluation of a heterogeneous non-antibody-producing population. Furthermore, it could be used for monitoring the changes in each immunoglobulin (Ig) chain content of the cells during the batch culture, which will facilitate the study on antibody synthesis, assembly and secretion.
Publisher
CHAPMAN HALL LTD
Issue Date
1996-08
Language
English
Keywords

HEPATITIS-B VIRUS; PRE-S2 SURFACE-ANTIGEN; SERUM-FREE MEDIA; CHIMERIC ANTIBODY; HYBRIDOMA CELLS; BATCH CULTURE; STABILITY; PRODUCTIVITY; IGG; SECRETION

Citation

BIOTECHNOLOGY TECHNIQUES, v.10, no.8, pp.615 - 620

ISSN
0951-208X
DOI
10.1007/BF00157372
URI
http://hdl.handle.net/10203/74794
Appears in Collection
BS-Journal Papers(저널논문)
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