Isolated human germinal center centroblasts have an intact mismatch repair system

Ig somatic hypermutation contributes to the generation of high-affinity Abs that are essential for efficient humoral defense, The presence of multiple point mutations in rearranged Ig V genes and their immediate flanking sequences suggests that the DNA repair system may not be working properly in correcting point mutations introduced to the restricted region of Ig genes. We examined the DNA repair functions of germinal center (GC) centroblasts, which are the cells in which ongoing Ig hypermutation takes place, We found that GC centroblasts express all known components of the human DNA mismatch repair system, and that the system corrects DNA mismatches in a strand-specific manner in vitro. We conclude that general suppression of mismatch repair at the cellular level does not occur during somatic hypermutation.
Publisher
AMER ASSOC IMMUNOLOGISTS
Issue Date
1998-12
Language
English
Keywords

SOMATIC HYPERMUTATION; IMMUNOGLOBULIN GENES; XERODERMA-PIGMENTOSUM; NUCLEAR EXTRACTS; TUMOR-CELLS; HETERODIMER; MUTATION; TONSIL; CANCER

Citation

JOURNAL OF IMMUNOLOGY, v.161, no.11, pp.6128 - 6132

ISSN
0022-1767
URI
http://hdl.handle.net/10203/74765
Appears in Collection
BS-Journal Papers(저널논문)
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