Production of L-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway

Cited 26 time in webofscience Cited 0 time in scopus
  • Hit : 490
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorPark, Hee-Sungko
dc.contributor.authorLee, JYko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2013-03-02T14:03:09Z-
dc.date.available2013-03-02T14:03:09Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1998-04-
dc.identifier.citationBIOTECHNOLOGY AND BIOENGINEERING, v.58, no.2-3, pp.339 - 343-
dc.identifier.issn0006-3592-
dc.identifier.urihttp://hdl.handle.net/10203/73876-
dc.description.abstractAs an alternative approach to the production of L-DOPA from a cheap raw material, we constructed a hybrid pathway consisting of toluene dioxygenase, toluene cis-glycol dehydrogenase, and tyrosine phenol-lyase. In this pathway, catechol is formed from benzene through the sequential action of toluene dioxygenase and toluene cis-glycol dehydrogenase, and L-DOPA is synthesized from the resulting catechol in the presence of pyruvate and ammonia by tyrosine phenol-lyase cloned from Citrobacter freundii. When the hybrid pathway was expressed in E. coil, production of L-DOPA was as low as 3 mM in 4 h due to the toxic effect of benzene on the cells. In order to reduce lysis of cells, Pseudomonas aeruginosa was employed as an alternative, which resulted in accumulation of about 14 mM L-DOPA in 9 h, showing a stronger resistance to benzene. (C) 1998 John Wiley & Sons, Inc.-
dc.languageEnglish-
dc.publisherJOHN WILEY SONS INC-
dc.subjectNUCLEOTIDE-SEQUENCE-
dc.subjectPSEUDOMONAS-PUTIDA-
dc.subjectESCHERICHIA-COLI-
dc.subjectTOLUENE-
dc.subjectCATECHOLS-
dc.titleProduction of L-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway-
dc.typeArticle-
dc.identifier.wosid000072158500036-
dc.identifier.scopusid2-s2.0-0032485910-
dc.type.rimsART-
dc.citation.volume58-
dc.citation.issue2-3-
dc.citation.beginningpage339-
dc.citation.endingpage343-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOENGINEERING-
dc.contributor.localauthorPark, Hee-Sung-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.nonIdAuthorLee, JY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorL-DOPA(3,4-dihydroxyphenyl-L-alanine)-
dc.subject.keywordAuthorhybrid pathway-
dc.subject.keywordAuthortyrosine phenol lyase-
dc.subject.keywordAuthortoluene dioxygenase-
dc.subject.keywordAuthorbenzene-
dc.subject.keywordPlusNUCLEOTIDE-SEQUENCE-
dc.subject.keywordPlusPSEUDOMONAS-PUTIDA-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusTOLUENE-
dc.subject.keywordPlusCATECHOLS-
Appears in Collection
CH-Journal Papers(저널논문)BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 26 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0