DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, C.D. | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-03-02T13:19:02Z | - |
dc.date.available | 2013-03-02T13:19:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-02 | - |
dc.identifier.citation | RNA, v.5, no.2, pp.290 - 301 | - |
dc.identifier.issn | 1355-8382 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73709 | - |
dc.description.abstract | The rat β-tropomyosin (β-TM) gene encodes both skeletal muscle β-TM mRNA and nonmuscle TM-1 mRNA via alternative RNA splicing. This gene contains eleven exons: exons 1-5, 8, and 9 are common to both mRNAs; exons 6 and 11 are used in fibroblasts as well as in smooth muscle, whereas exons 7 and 10 are used in skeletal muscle. Previously we demonstrated that utilization of the 3' splice site of exon 7 is blocked in nonmuscle cells. In this study, we use both in vitro and in vivo methods to investigate the regulation of the 5' splice site of exon 7 in nonmuscle cells. The 5' splice site of exon 7 is used efficiently in the absence of flanking sequences, but its utilization is suppressed almost completely when the upstream exon 6 and intron 6 are present. The suppression of the 5' splice site of exon 7 does not result from the sequences at the 3' end of intron 6 that block the use of the 3' splice site of exon 7. However, mutating two conserved nucleotides GU at the 5' splice site of exon 6 results in the efficient use of the 5' splice site of exon 7. In addition, a mutation that changes the 5' splice site of exon 7 to the consensus U1 snRNA binding site strongly stimulates the splicing of exon 7 to the downstream common exon 8. Collectively, these studies demonstrate that 5' splice site competition is responsible, in part, for the suppression of exon 7 usage in nonmuscle cells. | - |
dc.language | English | - |
dc.publisher | Cold Spring Harbor Laboratory Press | - |
dc.title | Donor site competition is involved in the regulation of alternative splicing of the rat β-tropomyosin pre-mRNA | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-0033035470 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 290 | - |
dc.citation.endingpage | 301 | - |
dc.citation.publicationname | RNA | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Chen, C.D. | - |
dc.subject.keywordAuthor | Cis-competition | - |
dc.subject.keywordAuthor | RNA processing | - |
dc.subject.keywordAuthor | Splice site selection | - |
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