DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sim, S | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Kim, H | ko |
dc.contributor.author | Ahn, JK | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.contributor.author | Cho, BR | ko |
dc.contributor.author | Park, I | ko |
dc.date.accessioned | 2013-03-02T12:53:50Z | - |
dc.date.available | 2013-03-02T12:53:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-03 | - |
dc.identifier.citation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.31, no.2, pp.177 - 182 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73610 | - |
dc.description.abstract | A pool of cis-acting hammerhead ribozymes randomized in their substrate recognition sequences was constructed. A variety of active cis-acting ribozymes which had various structures of stems I and III was selected from the pool by in vitro selection. The selected ribozymes were cloned and sequenced, The relationship between the cleavage efficiency and base-pairing in stems I and III of the selected ribozymes was investigated. The ribozymes with the smaller difference in folding energies between the active conformation and the stable but inactive conformation showed a tendency to have the better cleavage efficiency. The optimum length of stem I was 5 or 6 bases while the longer stem III, in general, appeared to be required for efficient cleavage. The specificity of the ribozyme reaction is discussed in terms of the length of stems I and III. | - |
dc.language | English | - |
dc.publisher | SPRINGER-VERLAG SINGAPORE PTE LTD | - |
dc.subject | HEPATITIS-B VIRUS | - |
dc.subject | CLEAVAGE RATES | - |
dc.subject | RNA | - |
dc.subject | SITE | - |
dc.title | In vitro selection of hammerhead ribozymes with optimized stems I and III | - |
dc.type | Article | - |
dc.identifier.wosid | 000073272600012 | - |
dc.identifier.scopusid | 2-s2.0-0032384310 | - |
dc.type.rims | ART | - |
dc.citation.volume | 31 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 177 | - |
dc.citation.endingpage | 182 | - |
dc.citation.publicationname | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Sim, S | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.contributor.nonIdAuthor | Kim, H | - |
dc.contributor.nonIdAuthor | Ahn, JK | - |
dc.contributor.nonIdAuthor | Cho, BR | - |
dc.contributor.nonIdAuthor | Park, I | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | hammerhead ribozyme | - |
dc.subject.keywordAuthor | hepatitis B virus | - |
dc.subject.keywordAuthor | in vitro selection | - |
dc.subject.keywordAuthor | substrate recognition sequence | - |
dc.subject.keywordPlus | HEPATITIS-B VIRUS | - |
dc.subject.keywordPlus | CLEAVAGE RATES | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | SITE | - |
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