DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong Ryoung | ko |
dc.contributor.author | Kang, Ke Won | ko |
dc.date.accessioned | 2013-02-28T06:28:22Z | - |
dc.date.available | 2013-02-28T06:28:22Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | European Journal of Biochemistry, v.254, no.3, pp.692 - 697 | - |
dc.identifier.issn | 0014-2956 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73248 | - |
dc.description.abstract | A serine-protease inhibitor of plasma kallikrein was screened and purified from a native Korean leech species, Hirudo nipponia. The peptide, named piguamerin, potently inhibited plasma and tissue kallikreins, and trypsin. Sequence analyses by automated Edman degradation revealed 48 amino acid residues and a molecular mass for the peptide of 5090 Da. Piguamerin is similar to antistasin-type inhibitors with the same spacing of ten cysteine residues, but shows differences from hirustasin, antistasin and ghilanten at the residues surrounding Arg27, which is a common P1 reactive residue for these inhibitors. The purified inhibitor modulated plasma clotting in tests of activated partial thromboplastin time at nanomolar concentrations. The serine-protease inhibitor of this leech may be involved in leech hematophagia. | - |
dc.language | English | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.title | Amino acid sequence of piguamerin, an antistasin-type protease inhibitor from the blood sucking leech Hirudo nipponia | - |
dc.type | Article | - |
dc.identifier.wosid | 000074472700031 | - |
dc.identifier.scopusid | 2-s2.0-0000339569 | - |
dc.type.rims | ART | - |
dc.citation.volume | 254 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 692 | - |
dc.citation.endingpage | 697 | - |
dc.citation.publicationname | European Journal of Biochemistry | - |
dc.identifier.doi | 10.1046/j.1432-1327.1998.2540692.x | - |
dc.contributor.nonIdAuthor | Kim, Dong Ryoung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | leech | - |
dc.subject.keywordAuthor | plasma kallikrein | - |
dc.subject.keywordAuthor | antistasin-type inhibitor | - |
dc.subject.keywordPlus | FACTOR-XA | - |
dc.subject.keywordPlus | GUAMERIN | - |
dc.subject.keywordPlus | COAGULATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | KALLIKREIN | - |
dc.subject.keywordPlus | ELASTASE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CLONING | - |
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