DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyoung Man | ko |
dc.date.accessioned | 2013-02-28T05:56:44Z | - |
dc.date.available | 2013-02-28T05:56:44Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v.328, no.1, pp.78 - 84 | - |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73128 | - |
dc.description.abstract | A mutant ribose binding protein (REP) of Escherichia coli was obtained by site directed mutagenesis, replacing Phe-187 in the wild-type REP (WT-REP) with a Trp residue, in order to compare its stability and folding behavior with those of the WT-REP, The equilibrium unfolding properties and the folding kinetics of these proteins were monitored by fluorescence and circular dichroism (CD), For both WT-REP and the Trp-substituted REP (Trp-REP), the conformational stabilities of the precursor proteins and the mature proteins were the same, indicating that the signal peptide had no influence on the property of the mature domain, The Phe/Trp substitution in the mature domain, however, brought about a significant decrease in the conformational stability, The signal peptide had an appreciable retarding effect on the folding of the precursor Trp-REP as was reported for the WT-REP, Refolding kinetics of the WT-REP and Trp-REP showed a two-step reaction when monitored by fluorescence and by CD. (C) 1996 Academic Press, Inc. | - |
dc.language | English | - |
dc.publisher | Elsevier Science Inc | - |
dc.subject | PHOSPHOGLYCERATE KINASE | - |
dc.subject | SIGNAL SEQUENCE | - |
dc.subject | PEPTIDES | - |
dc.subject | RESIDUES | - |
dc.title | Stability and folding of precursor and mature tryptophan-substituted ribose binding protein of Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UD39600011 | - |
dc.identifier.scopusid | 2-s2.0-0029870603 | - |
dc.type.rims | ART | - |
dc.citation.volume | 328 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 78 | - |
dc.citation.endingpage | 84 | - |
dc.citation.publicationname | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | ribose binding protein | - |
dc.subject.keywordAuthor | Trp-ribose binding protein | - |
dc.subject.keywordAuthor | unfolding equilibrium | - |
dc.subject.keywordAuthor | refolding kinetics | - |
dc.subject.keywordAuthor | domain folding | - |
dc.subject.keywordPlus | PHOSPHOGLYCERATE KINASE | - |
dc.subject.keywordPlus | SIGNAL SEQUENCE | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | RESIDUES | - |
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