Isolation of Genomic DNA Containing a Cytosolic Ascorbate Peroxidase Gene (ApxSC) from the Strawberry (Fragaria x ananassa)

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We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three start sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box.
Publisher
Japan Soc Biosci Biotechn Agrochem
Issue Date
1998-07
Language
English
Article Type
Article
Keywords

ARABIDOPSIS-THALIANA; MOLECULAR-CLONING; HYDROGEN-PEROXIDE; EXPRESSION; MAIZE; ELEMENT; INTRON; METABOLISM; SEQUENCE; SPINACH

Citation

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.62, no.7, pp.1358 - 1363

ISSN
0916-8451
URI
http://hdl.handle.net/10203/69838
Appears in Collection
BS-Journal Papers(저널논문)
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