Effects of enzyme concentration and film thickness on the analytical performance of a polypyrrole/glucose oxidase biosensor

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We describe the effect of preparation conditions during electropolymerization such as enzyme concentration in the deposition solution and film thickness on the analytical performance of the resulting polypyrrole / glucose oxidase (PPy/GOD) film. The responses of the resulting PPy/GOD biosensor to glucose and ascorbate were characterized and optimized. It was found that the enzyme concentration and the film thickness have the equivalent effects on the response of the resulting biosensor and that several optimal sets of the two parameters exist. The investigations regarding the response characteristic and the suppression of interference clearly indicate that the performance of biosensor could be significantly improved by making the film thick at low enzyme concentration.
Publisher
TAYLOR & FRANCIS INC
Issue Date
1995-01
Language
ENG
Article Type
Article
Keywords

ELECTROCHEMICAL IMMOBILIZATION; ELECTRODES; ELECTROPOLYMERIZATION; SELECTIVITY

Citation

ANALYTICAL LETTERS, v.28, no.6, pp.1017 - 1031

ISSN
0003-2719
URI
http://hdl.handle.net/10203/69786
Appears in Collection
BS-Journal Papers(저널논문)
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