Isolation of thermostable D-hydantoinase-producing thermophilic Bacillus sp SD-1

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A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1,000 thermophiles and identified to be Bacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60°C. The hydantoinase of Bacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70°C, respectively. The D-hydantoinase was stable upto 70°C, and half-life of the enzyme was about 20 min at 80°C.
Publisher
Springer Netherlands
Issue Date
1994-01
Language
ENG
Article Type
Article
Appears in Collection
BS-Journal Papers(저널논문)
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