DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D.M. | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-02-27T07:20:48Z | - |
dc.date.available | 2013-02-27T07:20:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1992 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.39, no.3, pp.336 - 342 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67208 | - |
dc.description.abstract | Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chitin-immobilized inulinase in alginate beads. The optimum amounts of both chitin-immobilized inulinase and permeabilized cells for coimmobilization were determined, and operational conditions were optimized. In a continuous stirred tank reactor operation, the maximum productivities for gluconic acid and sorbitol were about 19.2 and 21.3 g/L/h, respectively, at the dilution rate of 0.23 h-1 and the substrate concentration of 20%, but operational stability was low because of the abrasion of the beads. As an approach to increase the operational stability, a recycle packed-bed reactor (RPBR) was employed. In RPBR operation, the maximum productivities for gluconic acid and sorbitol were found to be 23.4 and 26.0 g/L/h, respectively, at the dilution rate of 0.35 h-1 and the substrate concentration of 20% when the recirculation rate was fixed at 900 mL/h. Coimmobilized enzymes were stable for 250 h in a recycle packed-bed reactor without any loss of activity, while half-life in a continuous stirred tank reactor (CSTR) was observed to be about 150 h. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.subject | FRUCTOSE | - |
dc.title | CONTINUOUS PRODUCTION OF GLUCONIC ACID AND SORBITOL FROM JERUSALEM-ARTICHOKE AND GLUCOSE USING AN OXIDOREDUCTASE OF ZYMOMONAS-MOBILIS AND INULINASE | - |
dc.type | Article | - |
dc.identifier.wosid | A1992HA00100011 | - |
dc.identifier.scopusid | 2-s2.0-0027112085 | - |
dc.type.rims | ART | - |
dc.citation.volume | 39 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 336 | - |
dc.citation.endingpage | 342 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.260390312 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kim, D.M. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FRUCTOSE | - |
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