FORMATION OF NASCENT INTERCALATED DISKS BETWEEN GRAFTED FETAL CARDIOMYOCYTES AND HOST MYOCARDIUM

Fetal cardiomyocytes isolated from transgenic mice carrying a fusion gene of the alpha-cardiac myosin heavy chain promoter with a beta-galactosidase reporter were examined for their ability to form stable intracardiac grafts. Embryonic day 15 transgenic cardiomyocytes delivered directly into the myocardium of syngeneic hosts formed stable graft, as identified by nuclear beta-galactosidase activity. Grafted cardiomyocytes were observed as long as 2 months after implantation, the latest date assayed. Intracardiac graft formation did not induce overtly negative effects on the host myocardium and was not associated with chronic immune rejection. Electron microscopy revealed the presence of nascent intercalated disks connecting the engrafted fetal cardiomyocytes and the host myocardium. These results suggest that intracardiac grafting might provide a useful approach for myocardial repair, provided that the grafted cells can contribute to myocardial function.
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Issue Date
1994-04
Language
ENG
Keywords

TRANSGENIC MICE; T-ANTIGEN; EXPRESSION; HYPERTROPHY; MYOCYTES; NEURONS; INVIVO; CELLS; GENES

Citation

SCIENCE, v.264, no.5155, pp.98 - 101

ISSN
0036-8075
DOI
10.1126/science.8140423
URI
http://hdl.handle.net/10203/67203
Appears in Collection
MSE-Journal Papers(저널논문)
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