DC Field | Value | Language |
---|---|---|
dc.contributor.author | CHUNG, BH | ko |
dc.contributor.author | SOHN, JH | ko |
dc.contributor.author | RHEE, SK | ko |
dc.contributor.author | Chang, YongKeun | ko |
dc.contributor.author | PARK, YH | ko |
dc.date.accessioned | 2013-02-27T06:48:25Z | - |
dc.date.available | 2013-02-27T06:48:25Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-01 | - |
dc.identifier.citation | JOURNAL OF FERMENTATION AND BIOENGINEERING, v.77, no.1, pp.75 - 79 | - |
dc.identifier.issn | 0922-338X | - |
dc.identifier.uri | http://hdl.handle.net/10203/67074 | - |
dc.description.abstract | Hirudin variants were constructed to exhibit an increased metal-binding affinity in an attempt to apply a metal-affinity partitioning process in a primary separation step for purification of hirudin. The hirudin variants were genetically engineered to contain additional surface-accessible histidines and produced by recombinant Saccharomyces cerevisiae. The partitioning behavior of these variants was compared with that of the wild type with a single surface-accessible histidine at position 51. Upon the addition of a small amount of Cu(II)IDA-PEG (Cu(II)iminodiacetic acid-polyethylene glycol) ligand to PEG/dextran two-phase systems, the hirudin variants with two or three surface-accessible histidines were more selectively partitioned into the PEG-rich phase than the wild type. Integrating protein engineering to metal-affinity partitioning offers the potential for general application of this technique to facilitate protein isolation, but the genetically engineered protein variants should be carefully constructed in a manner to minimize reduction of native protein activity. | - |
dc.language | English | - |
dc.publisher | SOC FERMENTATION BIOENGINEERING | - |
dc.subject | PROTEINS | - |
dc.subject | PURIFICATION | - |
dc.subject | SITE | - |
dc.title | ENHANCED METAL-AFFINITY PARTITIONING OF GENETICALLY-ENGINEERED HIRUDIN VARIANTS IN POLYETHYLENE-GLYCOL DEXTRAN 2-PHASE SYSTEMS | - |
dc.type | Article | - |
dc.identifier.wosid | A1994NC86700015 | - |
dc.identifier.scopusid | 2-s2.0-0028329210 | - |
dc.type.rims | ART | - |
dc.citation.volume | 77 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 75 | - |
dc.citation.endingpage | 79 | - |
dc.citation.publicationname | JOURNAL OF FERMENTATION AND BIOENGINEERING | - |
dc.contributor.localauthor | Chang, YongKeun | - |
dc.contributor.nonIdAuthor | CHUNG, BH | - |
dc.contributor.nonIdAuthor | SOHN, JH | - |
dc.contributor.nonIdAuthor | RHEE, SK | - |
dc.contributor.nonIdAuthor | PARK, YH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | SITE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.