DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Yeon-Soo | ko |
dc.contributor.author | Muller, Friedemann | ko |
dc.contributor.author | Lusky, Monika | ko |
dc.contributor.author | Hurwitz, Jerard | ko |
dc.date.accessioned | 2013-02-25T22:10:02Z | - |
dc.date.available | 2013-02-25T22:10:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1993-01 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.90, no.2, pp.702 - 706 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/65660 | - |
dc.description.abstract | Replication of bovine papilloma virus (BPV) DNA requires two virus-encoded proteins, E1 and E2, while all other proteins are supplied by the host cell. Here, we describe the isolation of the E1 protein and show that it is a multifunctional protein. Purified E1 protein was required for the in vitro replication of BPV origin-containing DNA by extracts of mouse cells, as reported [Yang, L., Li, R., Mohr, I. J., Clark, R. & Botchan, M. R. (1991) Nature (London) 353, 628-632]. In addition, the E1 protein cosedimented with a number of other activities including (i) DNA helicase activity, (ii) BPV origin-containing DNA-specific binding activity, (iii) DNA-dependent ATPase activity, and (iv) BPV origin-specific unwinding of superhelical DNA. The E1 protein, acting as a helicase, moved in the 3'--> 5' direction, like simian virus 40 (SV40) large tumor antigen, which plays a pivotal role in SV40 DNA replication. However, unlike the SV40 large tumor antigen, the helicase activity of E1 was stimulated 5-fold by the presence of a fork structure at the junction between single-stranded and double-stranded DNA and was supported efficiently by all eight nucleoside triphosphates. The E1-catalyzed ATPase activity required the presence of single-stranded or double-stranded DNAs. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | T-ANTIGEN | - |
dc.subject | BINDING PROTEIN | - |
dc.subject | VIRAL ORIGIN | - |
dc.subject | SV40 ORIGIN | - |
dc.subject | E2 | - |
dc.subject | COMPLEX | - |
dc.subject | INVITRO | - |
dc.subject | CELLS | - |
dc.title | Bovine papilloma virus (BPV)-encoded E1 protein contains multiple activities required for BPV DNA replication | - |
dc.type | Article | - |
dc.identifier.wosid | A1993KH51600072 | - |
dc.identifier.scopusid | 2-s2.0-0027402449 | - |
dc.type.rims | ART | - |
dc.citation.volume | 90 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 702 | - |
dc.citation.endingpage | 706 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.90.2.702 | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.nonIdAuthor | Muller, Friedemann | - |
dc.contributor.nonIdAuthor | Lusky, Monika | - |
dc.contributor.nonIdAuthor | Hurwitz, Jerard | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DNA HELICASE | - |
dc.subject.keywordAuthor | HIGHLY UNWOUND SUPERHELICAL DNA | - |
dc.subject.keywordAuthor | ORIGIN BINDING | - |
dc.subject.keywordPlus | T-ANTIGEN | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
dc.subject.keywordPlus | VIRAL ORIGIN | - |
dc.subject.keywordPlus | SV40 ORIGIN | - |
dc.subject.keywordPlus | E2 | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | CELLS | - |
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