Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors

The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Issue Date
1994-09
Language
ENG
Keywords

PRE-MESSENGER-RNA; PREMESSENGER RNA; NONMUSCLE CELLS; SITE SELECTION; SR PROTEINS; GENE; INVITRO; EXPRESSION; EXON; CIS

Citation

SCIENCE, v.265, no.5179, pp.1706 - 1709

ISSN
0036-8075
DOI
10.1126/science.8085156
URI
http://hdl.handle.net/10203/64800
Appears in Collection
BS-Journal Papers(저널논문)
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