CONTROL OF DISSOLVED OXYGEN CONCENTRATION IN MAMMALIAN CELL CULTURE USING A COMPUTER-COUPLED MASS FLOW CONTROLLER

A simple proportional control system for dissolved oxygen(DO) concentration in cell culture medium was developed by using a computer-coupled mass flow controller. The DO levels were very stable during the cultivation of Vero-6, while flow rates of air and/or oxygen enriched air were gradually changed depending on the DO concentration and the preset DO level. Vero-6 cells could grow normally to the confluence in the range of 30% and 50% of DO. Growth of Veto-6 at 10% of DO was markedly retarded.
Publisher
SPRINGER
Issue Date
1992-09
Language
ENG
Citation

BIOTECHNOLOGY TECHNIQUES, v.6, no.5, pp.405 - 408

ISSN
0951-208X
URI
http://hdl.handle.net/10203/63720
Appears in Collection
BS-Journal Papers(저널논문)
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