DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, YS | ko |
dc.contributor.author | LEE, HB | ko |
dc.contributor.author | CHOI, KD | ko |
dc.contributor.author | PARK, S | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.date.accessioned | 2008-07-21T02:43:19Z | - |
dc.date.available | 2008-07-21T02:43:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-01 | - |
dc.identifier.citation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.58, no.1, pp.111 - 116 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | http://hdl.handle.net/10203/6156 | - |
dc.description.abstract | A gene (estC) coding for an esterase (esterase III) of Pseudomonas fluorescens was cloned into Escherichia coli JM83. DNA sequencing showed a single open reading frame with GTG as a translation initiation codon for esterase III. This was confirmed by N-terminal amino acid sequence analysis of the purified esterase III protein from an E. coli clone. The promoter sequence and a potential Shine-Dalgarno sequence were followed by the coding sequence of the estC gene. The amino acid sequence deduced from the nucleotide sequence contains the consensus active site sequence, G-X-S-X-G, of serine esterase. The esterase III expressed in an E. coli clone was purified by ion-exchange chromatography and gel filtration. The native form of the enzyme was a monomer with a molecular weight of 41,000. The results of substrate specificity and the inhibitor studies suggest that this enzyme is a carboxylesterase (EC 3.1.1.1) and a serine residue is present at the active site of the enzyme. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JAPAN SOC BIOSCI BIOTECHN AGROCHEM | - |
dc.subject | TRANSLATIONAL INITIATION | - |
dc.subject | INSECTICIDE RESISTANCE | - |
dc.subject | INTRACELLULAR ESTERASE | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | PURIFICATION | - |
dc.subject | LIPASE | - |
dc.subject | MEMBRANE | - |
dc.subject | PROTEINS | - |
dc.title | CLONING OF PSEUDOMONAS-FLUORESCENS CARBOXYLESTERASE GENE AND CHARACTERIZATION OF ITS PRODUCT EXPRESSED IN ESCHERICHIA-COLI | - |
dc.type | Article | - |
dc.identifier.wosid | A1994MU66800024 | - |
dc.identifier.scopusid | 2-s2.0-85007980736 | - |
dc.type.rims | ART | - |
dc.citation.volume | 58 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 111 | - |
dc.citation.endingpage | 116 | - |
dc.citation.publicationname | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | KIM, YS | - |
dc.contributor.nonIdAuthor | LEE, HB | - |
dc.contributor.nonIdAuthor | CHOI, KD | - |
dc.contributor.nonIdAuthor | PARK, S | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TRANSLATIONAL INITIATION | - |
dc.subject.keywordPlus | INSECTICIDE RESISTANCE | - |
dc.subject.keywordPlus | INTRACELLULAR ESTERASE | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | LIPASE | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PROTEINS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.