DNA light-strand preferential recognition of human mitochondria transcription termination factor mTERF

Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTGTGGCA GANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-TGGCAGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity.
Publisher
SPRINGER SINGAPORE PTE LTD
Issue Date
2005-11
Language
ENG
Description

http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=173&mid=9&pid=9

Keywords

TRANSFER RNALEU(UUR) GENE; POINT MUTATION; MELAS SUBGROUP; SEQUENCE; BINDING; ENCEPHALOMYOPATHIES; INVITRO; PROTEIN

Citation

JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.38, no.6, pp.690 - 694

ISSN
1225-8687
URI
http://hdl.handle.net/10203/6027
Appears in Collection
BS-Journal Papers(저널논문)
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