EFFECT OF MECHANICAL AGITATION ON HYBRIDOMA CELL-GROWTH

Hybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr −1 in T flasks but 0.018 hr −1 and 0.028 hr −1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr −1 for all cases.
Publisher
Springer Netherlands
Issue Date
1988-12
Language
ENG
Citation

BIOTECHNOLOGY LETTERS, v.10, no.9, pp.625 - 628

ISSN
0141-5492
DOI
10.1007/BF01024713
URI
http://hdl.handle.net/10203/57765
Appears in Collection
BS-Journal Papers(저널논문)
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