I. Functionalization of Cellulose Diacetate Fibers by Photografting of Cyclodextrin Derivatized with an Aryl Azide Group
In this paper, a UV-reactive cyclodextrin derivative, N-(6,9,12-trioxa-azapentadecan-15-yl)-4-azido-2-hydroxybenzaido-β-cyclodextrin (Azide-CD), was successfully grafted onto cellulose diacetate (CDA) fibers. The Azide-CD was adsorbed onto CDA fibers by dip-coating, and then UV light was irradiated on the Azide-CD-coated CDA fibers for the chemical fixation of the cyclodextrin moiety. The effect of the concentration of Azide-CD in solution was investigated. The grafting amount of Azide-CD increased as the concentration of Azide-CD in solution increased from 0.5 to 2 mM. CDA fibers grafted with cyclodextrin were characterized by Fourier transform infrared spectroscopy (FT-IR). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to show the morphology changes of the fiber surfaces. The complexation ability of the grafted cyclodextrin was demonstrated by fluorescence microscopy using coumarin dye, known to bind strongly with β-cyclodextrin, as a model guest molecule.
II. Studies on Cell-Including Hydrogel Matrices for Development of Advanced Wound Dressings
Hydrogels have been widely used in biomedical areas, such as tissue engineering and drug delivery, due to their biocompatibility, flexibility, and wettability. In this study, the acrylic acid-based hydrogel was used for the formation of cell-including polymer matrices. The polymeric hydrogel matrices were prepared by swelling the hydrogel in water. The cells were injected into the hydrogel matrices, and the viability of cells included inside the hydrogel matrices was analyzed. For the analysis of cell viability, the kidney epithelial 293 cells were stained with 4′,6-diamidino-2-phenylindole (DAPI). The kidney epithelial 293 cells, transfected with the green fluorescent protein (GFP)-expressing construct, were also used. The persistent expression of GFP w...