A biofunctionalized viral delivery patch for spatially defined transfection

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Gene therapy holds the significance of correcting genetic defects. However, difficulties in the in vivo delivery to the targeted tissues and systemic delivery remain the biggest challenges to be overcome. Here, a robust system of biofunctionalized polymeric layermediated lentiviral delivery was designed for the site-specific spatial and temporal control of viral gene delivery. Poly glycidyl methacrylate (pGMA) modification of a substrate via initiated chemical vapor deposition (iCVD) followed by polyethyleneimine (PEI) immobilization provided the adhesion site for the lentivirus. Furthermore, the polymeric patch based gene delivery system showed a high rate of gene transduction compared to bolus treatment. Furthermore, by using mask patterning, we were able to spatially pattern the lentivirus which allowed spatially defined transfection.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2019-02
Language
English
Article Type
Article
Citation

CHEMICAL COMMUNICATIONS, v.55, no.16, pp.2317 - 2320

ISSN
1359-7345
DOI
10.1039/c8cc09768b
URI
http://hdl.handle.net/10203/268940
Appears in Collection
CBE-Journal Papers(저널논문)
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