Lissajous fiber scanning based two-photon endomicroscope for In vivo tissue imaging = 생체 조직 영상용 리사쥬 스캐닝 기반 이광자 내시 현미경

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We report a Lissajous two-photon endomicroscope for in vivo tissue imaging. The Lissajous fiber scanner is composed of a piezoelectric (PZT) tube, a double-clad fiber (DCF), and micro-tethered-silicon-oscillators (MTSO), attached on the DCF to generate Lissajous scanning. The Lissajous scanning achieved 5 frames/s with 89.5 % of scanning density by using the frequency selection rule. The endoscopic scanner was compactly packaged with 2.6 mm in diameter including a high NA gradient-index (GRIN) lens. The endomicroscope provides 0.70 µm resolution with 1 µm diameter fluorescent beads. Two-photon fluorescence images of a stained kidney section and blood vessels and nucleus with green fluorescent protein (GFP) from anesthetized mice are successfully obtained by using the packaged endomicroscope. The Lissajous two-photon endomicroscope can be a promising compact handheld platform for in vivo tissue imaging and surgical application.
Advisors
Jeong, Ki-Hunresearcher정기훈researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2018.8,[x, 54 p. :]

Keywords

내시 현미경▼a이광자 현미경▼a공진구동 광섬유 스캐너▼a리사쥬 패턴▼a생체 내 이미징; Endomicroscope▼atwo-photon microscope▼aresonant fiber scanner▼alissajous pattern▼ain vivo imaging

URI
http://hdl.handle.net/10203/266184
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=828491&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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