Role of phosphatidylinositol 4-phosphate and its effector proteins in neurotransmitter release = 신경전달물질 배출에서 phosphatidylinositol 4-phosphate와 하위 이펙터 단백질의 역할

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Phosphoinositides are minor components of cellular membrane, but serve crucial roles in cell physiology through the recruitment of effector proteins based on electrostatic interaction. Phosphatidylinositol 4-phosphate [PI4P], in recent, has emerged as an important regulator of diverse cellular process at the plasma membrane. However, in neuronal exocytosis, its own roles beyond being a synthetic precursor of phosphatidylinositol 4,5-bisphosphatate [PI(4,5)$P_2$] is unknown. Here, using structured illumination microscopy on the plasma membrane of PC12 cells from Rattus norvegicus, we show that PI4P is an inner-leaflet lipid that clusters in microdomains and that is involved in clustering of t-SNARE protein syntaxin 1, a protein essential for vesicle docking. Furthermore, we found that the syntaxin 1?associated exocytic proteins synaptotagmin 1 and synaptotagmin-like protein 4 interact with PI4P independently of $Ca^{2+}$ through their C2 domains at the plasma membrane. These electrostatic protein-lipid interaction is required for recruiting vesicles to the plasma membrane. Our results demonstrate that PI4P contribute to the anionic lipid pools that defines plasma membrane identify, directly regulate vesicle docking through electrostatic interaction with syntaxin 1 and C2 domain-contained exocytic proteins
Advisors
Heo, Won Doresearcher허원도researcher
Description
한국과학기술원 :나노과학기술대학원,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 나노과학기술대학원, 2017.8,[v, 63 p. :]

Keywords

phosphatidylinositol 4-phosphate▼avesicle docking▼aexocytosis▼asynapse▼aC2 domain; 포스파티딜이노시톨 4-포스페이트▼a소포 도킹▼a세포 외 유출▼a시냅스▼aC2 도메인

URI
http://hdl.handle.net/10203/264609
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=866900&flag=dissertation
Appears in Collection
NT-Theses_Ph.D.(박사논문)
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