DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Byung Chul | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2019-06-28T05:10:19Z | - |
dc.date.available | 2019-06-28T05:10:19Z | - |
dc.date.created | 2019-06-20 | - |
dc.date.created | 2019-06-20 | - |
dc.date.created | 2019-06-20 | - |
dc.date.created | 2019-06-20 | - |
dc.date.issued | 1993-11 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.42, no.9, pp.1107 - 1112 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/262852 | - |
dc.description.abstract | The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial cell bleeding. In the TCRC, fed by 50 g/L of glucose, a spore concentration of 1.6 x 10(10) CFU/mL was obtained with a spore percentage of greater than 95% and a maximum cell mass of 82.2 g/L. The volumetric productivity was four times higher than that obtained from batch cultivation. In the two-stage continuous culture with partial cell bleeding spore concentration was strongly dependent on the bleed ratio. The spore concentration of 1.8 x 10(9) CFU/mL and the spore percentage of 70% were obtained at the second stage when a bleed ratio of 0.33 and a dilution rate of 0.23 h-1 were used. (C) 1993 John Wiley & Sons, Inc. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.title | Production of Bacillus thuringiensis spores in total cell retention culture and two stage continuous culture using an internal ceramic filter system | - |
dc.type | Article | - |
dc.identifier.wosid | A1993MC12100012 | - |
dc.identifier.scopusid | 2-s2.0-0027910078 | - |
dc.type.rims | ART | - |
dc.citation.volume | 42 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 1107 | - |
dc.citation.endingpage | 1112 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.260420913 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Kang, Byung Chul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | BACILLUS-THURINGIENSIS | - |
dc.subject.keywordAuthor | SPORE | - |
dc.subject.keywordAuthor | INTERNAL MEMBRANE FILTER | - |
dc.subject.keywordAuthor | 2-STAGE CONTINUOUS CULTURE | - |
dc.subject.keywordPlus | FED-BATCH CULTURE | - |
dc.subject.keywordPlus | MEMBRANE | - |
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