In vivo longitudinal depth-wise visualization of tumorigenesis by needle-shaped side-view confocal endomicroscopy

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In vivo, longitudinal observation of tumorigenesis in a live mouse model over an extended time period has been actively pursued to obtain a better understanding of the cellular and molecular mechanism in a highly complex tumor microenvironment. However, common intravital imaging approaches based on a conventional laser scanning confocal or a two-photon microscope have been mostly limited to the observation of superficial parts of the solid tumor tissue. In this work, we implemented a small diameter needle-shaped side-view confocal endomicroscope that can be directly inserted into a solid tumor in a minimally-invasive manner in vivo. By inserting the side-view endomicroscope into the breast tumor from the surface, we achieved in vivo depth-wise cellular-level visualization of microvasculature and fluorescently labeled tumor cells located deeply inside the tumor. In addition, we successfully performed longitudinal depth-wise visualization of a growing breast tumor over three weeks in a live mouse model, which revealed dynamic changes in microvasculature such as a decreasing amount of intratumoral blood vessels over time. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Publisher
OPTICAL SOC AMER
Issue Date
2019-06
Language
English
Article Type
Article
Citation

BIOMEDICAL OPTICS EXPRESS, v.10, no.6, pp.2719 - 2729

ISSN
2156-7085
DOI
10.1364/BOE.10.002719
URI
http://hdl.handle.net/10203/262810
Appears in Collection
NT-Journal Papers(저널논문)
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