DC Field | Value | Language |
---|---|---|
dc.contributor.author | Miller, Brian C. | - |
dc.contributor.author | Zhao, Zijiang | - |
dc.contributor.author | Stephenson, Linda M. | - |
dc.contributor.author | Cadwell, Ken | - |
dc.contributor.author | Pua, Heather H. | - |
dc.contributor.author | Lee, Heung Kyu | - |
dc.contributor.author | Mizushima, Noboru | - |
dc.contributor.author | Iwasaki, Akiko | - |
dc.contributor.author | He, You-Wen | - |
dc.contributor.author | Swat, Wojciech | - |
dc.contributor.author | Virgin IV, Herbert W. | - |
dc.date.accessioned | 2011-09-07T04:26:47Z | - |
dc.date.available | 2011-09-07T04:26:47Z | - |
dc.date.issued | 2008-04-01 | - |
dc.identifier.citation | Autophagy, Vol.4, No.3, pp.23-29 | en |
dc.identifier.issn | 1554-8627 | - |
dc.identifier.uri | http://hdl.handle.net/10203/25121 | - |
dc.description.abstract | Macroautophagy (herein autophagy) is an evolutionarily conserved process, requiring the gene ATG5, by which cells degrade cytoplasmic constituents and organelles. Here we show that ATG5 is required for efficient B cell development and for the maintenance of B-1a B cell numbers. Deletion of ATG5 in B lymphocytes using Cre-LoxP technology or repopulation of irradiated mice with ATG5-/- fetal liver progenitors resulted in a dramatic reduction in B-1 B cells in the peritoneum. ATG5-/- progenitors exhibited a significant defect in B cell development at the pro- to pre-B cell transition, although a proportion of pre-B cells survived to populate the periphery. Inefficient B cell development in the bone marrow was associated with increased cell death, indicating that ATG5 is important for B cell survival during development. In addition, B-1a B cells require ATG5 for their maintenance in the periphery. We conclude that ATG5 is differentially required at discrete stages of development in distinct, but closely related, cell lineages. | en |
dc.description.sponsorship | This work was supported by NIH grants CA74730 and U54 AI057160 Project 6 to H.W.V.; AI06107703 and AI06302402 to W.S.; 5-T32-AI07163-27 to L.M.S.; CA92123 to Y.W.H.; AI064705 to A.I.; Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Toray Science Foundation to N.M. H.K.L. is a recipient of the Anna Fuller Fellowship. | en |
dc.language.iso | en_US | en |
dc.publisher | Landes Bioscience | en |
dc.subject | B cells | en |
dc.subject | cell differentiation and development | en |
dc.subject | transgenic/knockout mice | en |
dc.subject | ATG5 | en |
dc.title | The autophagy gene ATG5 plays an essential role in B lymphocyte development | en |
dc.type | Article | en |
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